Journal: Cells

Article Title: New Insights into the Pivotal Role of Iron/Heme Metabolism in TLR4/NF-κB Signaling-Mediated Inflammatory Responses in Human Monocytes

doi: 10.3390/cells10102549

Figure Lengend Snippet: Heme biosynthesis occurs during inflammation. ( A ) Heme assay showing the relative heme level obtained in THP-1 cells after treatment with LPS (10 ng/mL) for 48 h and mannitol or glucose (5.5 and 25 mM) for 72 h. Data obtained after three experiments. *** p < 0.001, control vs. LPS ( t -test). *** p < 0.001, mannitol vs. high glucose (ANOVA test). # The mean difference is significant at the 0.001 level. ( B ) Western blotting of MFRN, ABCB6, ABCB10, ALAS1, HO-1, FECH, and FLVCR1 proteins in THP-1 cells after treatment with LPS (10 ng/mL) for 48 h and mannitol or glucose (5.5 and 25 mM) for 72 h. Expression levels were estimated by densitometry and normalized to β-actin. Data were repeated thrice for confirmation. ( C ) Graphical representation of MFRN, ABCB6, ABCB10, ALAS1, HO-1, FECH, and FLVCR1 proteins in THP-1 cells after treatment with LPS (10 ng/mL) for 48 h. *** p < 0.001 ( t -test). Representative expression of proteins in THP-1 cells after treatment with mannitol or glucose (5.5 and 25 mM) for 72 h. All data were repeated three times for confirmation. *** p < 0.001 (ANOVA test). # p < 0.001 vs. control.

Article Snippet: Finally, an antibody specific to ABCB6 (PA1723) was obtained from Boster Bio (Pleasanton, CA, USA).

Techniques: Control, Western Blot, Expressing

Journal: Scientific Reports

Article Title: Comprehensive analysis of copper-metabolism-related genes about prognosis and immune microenvironment in osteosarcoma

doi: 10.1038/s41598-023-42053-w

Figure Lengend Snippet: Summary of descriptive characteristics of the included articles (n = 8).

Article Snippet: Then, the slides were hatched with COX11 primary antibody (11498-1-AP, proteintech, China), AP1B1 (R160051, ZENBIO, China) and ABCB6 primary antibody (R121438, ZENBIO, China) which were diluted into 1:100, at room temperature overnight.

Techniques: Binding Assay

Journal: Scientific Reports

Article Title: Comprehensive analysis of copper-metabolism-related genes about prognosis and immune microenvironment in osteosarcoma

doi: 10.1038/s41598-023-42053-w

Figure Lengend Snippet: Expression validation of CMRGs ( A ) Multivariate Cox regression analysis of prognostic CMRGs. ( B ) Kaplan–Meier plot of COX11, AP1B1, and ABCB6. ( C ) Immunohistochemical staining of COX11, AP1B1, and ABCB6.

Article Snippet: Then, the slides were hatched with COX11 primary antibody (11498-1-AP, proteintech, China), AP1B1 (R160051, ZENBIO, China) and ABCB6 primary antibody (R121438, ZENBIO, China) which were diluted into 1:100, at room temperature overnight.

Techniques: Expressing, Immunohistochemistry, Staining